| No | Size | Price | Availability | |
| ARM2353P-21 | 50ul | 1500.00 | In Stock |  |
| ARM2353P-21 | 100ul | 2000.00 | In Stock | |
| Source: | Rabbit | Gene Id: | 2353 |
| Isotype: | IgG | Swiss Prot: | P01100 |
| purity: | Affinity purification |
| Background: |
| The Fos family of nuclear oncogenes includes c-Fos, FosB, Fos-related antigen 1 (FRA1), and Fos-related antigen 2 (FRA2) (1). While most Fos proteins exist as a single isoform, the FosB protein exists as two isoforms: full-length FosB and a shorter form, FosB2 (Delta FosB), which lacks the carboxy-terminal 101 amino acids (1-3). The expression of Fos proteins is rapidly and transiently induced by a variety of extracellular stimuli including growth factors, cytokines, neurotransmitters, polypeptide hormones, and stress. Fos proteins dimerize with Jun proteins (c-Jun, JunB, and JunD) to form Activator Protein-1 (AP-1), a transcription factor that binds to TRE/AP-1 elements and activates transcription. Fos and Jun proteins contain the leucine-zipper motif that mediates dimerization and an adjacent basic domain that binds to DNA. The various Fos/Jun heterodimers differ in their ability to transactivate AP-1 dependent genes. In addition to increased expression, phosphorylation of Fos proteins by Erk kinases in response to extracellular stimuli may further increase transcriptional activity (4-6). Phosphorylation of c-Fos at Ser32 and Thr232 by Erk5 increases protein stability and nuclear localization (5). Phosphorylation of FRA1 at Ser252 and Ser265 by Erk1/2 increases protein stability and leads to overexpression of FRA1 in cancer cells (6). Following growth factor stimulation, expression of FosB and c-Fos in quiescent fibroblasts is immediate, but very short-lived, with protein levels dissipating after several hours (7). FRA1 and FRA2 expression persists longer, and appreciable levels can be detected in asynchronously growing cells (8). Deregulated expression of c-Fos, FosB, or FRA2 can result in neoplastic cellular transformation; however, Delta FosB lacks the ability to transform cells (2,3). |
| Reactivity | Human, Mouse, Rat |
| Tested applications | WB IP IF ChIP FC |
| Clonality | Monoclonal Antibody |
| Calculated MW | 62 kDa |
| Recommended Dilutions |
WB 1:1000
IF 1:800
FC 1:800
ChIP 1:50
Dot 1:50
|
| Immunogen | A synthetic phosphopeptide corresponding to Ser32 of human c-Fos protein |
| Storage | Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles. |
| Synonym | FOS, AP-1, C-FOS, Proto-oncogene c-Fos |
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