Home > FAQs > FAQs
  • The best way to find a specific product that fulfills all your research requirements is go about searching a product is to start by entering the antigen name, GENE ID or product Code. By the way, for searching it is not relevant if you use lower or upper case letters.

  • You can reduce the amount of found products by setting filters, which you may find next to the search results.
    For example, searching for antibodies you may select the needed reactivity (species), clonality of the antibody, what application you plan to perform, what host the antibody should be produced in and many more. The filter selection reduces the amount of products significantly to the ones that are most suitable for you.

  • While it has no influence if you use upper- or lower case letters the spelling can be crucial when searching. When using Greek letters we recommend trying different spelling. The prefix ‘alpha-’ for instance can also be used as ‘a-’.

  • If you cannot find the product you are looking for our customer support helps you. Please contact us using the phone number +86 021-54651191 or E-mail address: [email protected]

  • Our customer service will help you in case you need additional information of a product or the application of a product. Please contact us by phone or email:
    Phone +86 021-54651191
    E-Mail: [email protected]

  • In case you have added the wrong product in the Submitted shopping-basket, you must contact with us via phone or E-mail instantly and remove the wrong product.
  • After adding the product that interests you to the shopping basket you can increase the ordering amount by repeatedly clicking ‘add to shopping basket’. Another option is to send us a list of the number you want via E-mail: [email protected]
  • If you have a user account you are able to change your customer profile by yourself. You can create one billing address and multiple shipping addresses. In case you have added the wrong shipping-address in the Submitted shopping-basket, you must contact with us via phone or E-mail instantly and remove the wrong product.
  • We make sure that each product is being shipped in accordance to its individual requirements. Usually express shipment is being selected in order to avoid any impact on product quality. Of course we use cooling packs or dry-ice as needed.
  • If the product is in stock the products usually leave the manufacturer within two days after order confirmation.
  • We cooperate with a range of well established logistics companies like ZTO, S.F. Express, EMS and others. In case you prefer shipment via a courier of your choice, please contact our ordering department. We gladly help you with the planning process: [email protected], Phone +86 021-54651191. Of course, no shipping costs apply for courier shipments paid by you.
  • Shipping time depends on the product (In Stock or Future). The average shipping time is no long than two weeks for all products. The product state could be find on the web, extra information could be get from Yihyson via E-mail or phone.
  • We usually suggest customers using PVDF membranes rather than nitrocellulose membranes, because PVDF membranes offer better protein retention, physical strength and broad chemical compatibility. The typical binding capacity of commercially available PVDF membranes is 100-200 μg/cm2, compared to that of nitrocelllulose membranes having a binding capacity of 80-100 μg/cm2. However, if the detection requires that the transferred protein retains its native conformation after transfer, for instance, when the antibody recognizes 3-D structure (e.g. an antigenic epitope consisting of non-contiguous residues), nitrocellulose membranes might be preferred.
  • The concentration of antigen may be too low. If the relative concentration of the antigen of interest is too low (less than 0.2 % of total protein), it may be difficult to detect. If this happens please load more sample, or enrich the antigen by fractionation or by immunoprecipitation.
  • There are many factors that are responsible for additional bands appearing in western blot results:
    a) Mechanical degradation of the lysate: If samples are stored for a prolonged period or if proteins or membranes are fractionated after homogenization of the starting tissue, additional bands which are faster migrating than the targeted one may appear. To get around this use fresh lysate.
    b) Proteolytic degradation of the lysate: Proteolysis is a common cause of the appearance of background bands. Make sure there are sufficient protease inhibitors in the sample.
    c) Too much protein sample was loaded
    d) The detection system used was too sensitive
    e) The western blot membrane was not blocked correctly.
    f) Homologous proteins or other isoforms of the target protein: Around one third of proteins belong to protein families, containing homologous proteins which may cross-react with antibodies. Furthermore, one single gene can be translated into isoforms with different molecular weights. Antibodies usually can detect more than one of them. 
    g) Unspecificity of the antibody: Although Proteintech applies the most stringent quality assurance standards to ensure that our antibodies do not bind multiple targets; it is possible that the antibody binds some unrelated proteins, especially on those lysates which we did not test.
  • Please keep in mind that the predicted molecular weight is calculated from the protein sequence. There are several possibilities which may explain this observation:
    a) Degradation of the lysate: The sample may have been degraded by proteolysis; explaining why it will show a band with a different molecular weight than the one predicted from the sequence.
    b) Protein modification: Some proteins, such as membrane proteins, are commonly modified (e.g. glycosylation) after translation. This may result in the protein having a different molecular weight to that predicted from the primary protein sequence.
  • Yes, we can offer you a negative tissue control.
  • We would be pleased to investigate this for you. Please contact us for more detailed information.

service center
Contact Us