EIF2B5 Rabbit Monoclonal Antibody
No Size Price Availability  
ARM8893-11 50ul 1500.00 In Stock
ARM8893-11 100ul 2000.00 In Stock
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Source: Rabbit Gene Id: 8893
Isotype: IgG Swiss Prot: Q13144
purity: Affinity purification
Background:
Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. eIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex. eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B. Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51.eIF2B, a guanine nucleotide exchange factor, is composed of 5 subunits, the largest of which is eIF2B-epsilon (7). Multiple in vivo phosphorylation sites have been identified on eIF2B-epsilon (8). Casein Kinase II can phosphorylate eIF2B-epsilon at Ser717/718 to allow for association with its substrate eIF2. Phosphorylation at Ser544 allows GSK-3 to phosphorylate the key regulatory site Ser540. A fifth eIF2B-epsilon phosphorylation site, Ser466, can be phosphorylated by casein kinase I.
Reactivity Human
Tested applications WB ELISA
Clonality Monoclonal Antibody
Calculated MW 85 kDa
Recommended Dilutions
Immunogen A synthetic peptide of human EIF2B5 is used for rabbit immunization
Storage Store at -20°C or lower in PBS(pH 7.4). Avoid freeze/thaw cycles.
Synonym CACH, CLE, EIF-2B, EIF2Bepsilon, LVWM
This product is for research use only, not for diagnostic or therapeutic use!

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