Source: | Rabbit | Gene Id: | 142 |
Isotype: | IgG | Swiss Prot: | P09874 |
purity: | Affinity purification |
Background: |
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). |
Reactivity | Human, Monkey |
Tested applications | WB |
Clonality | Monoclonal Antibody |
Calculated MW | 89 kDa |
Recommended Dilutions |
WB 1:1000
|
Immunogen | A synthetic peptide corresponding to residues surrounding Asp214 in human PARP protein |
Storage | Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles. |
Synonym | PARP, PPOL, ADPRT, ARTD1, ADPRT1, PARP-1, ADPRT 1, pADPRT-1 |
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