ALK Rabbit Monoclonal Antibody (PE Conjugate)
No Size Price Availability  
ARM238-24C 50ul 1500.00 In Stock
ARM238-24C 100ul 2000.00 In Stock
  • Details
  • FAQs
  • Manual
Source: Rabbit Gene Id: 238
Isotype: IgG Swiss Prot: Q9UM73
purity: Affinity purification
Background:
Anaplastic lymphoma kinase (ALK) is a tyrosine kinase receptor for pleiotrophin (PTN), a growth factor involved in embryonic brain development (1-3). In ALK-expressing cells, PTN induces phosphorylation of both ALK and the downstream effectors IRS-1, Shc, PLCγ, and PI3 kinase (1). ALK was originally discovered as a nucleophosmin (NPM)-ALK fusion protein produced by a translocation (4). Investigators have found that the NPM-ALK fusion protein is a constitutively active, oncogenic tyrosine kinase associated with anaplastic lymphoma (4). Research literature suggests that activation of PLCγ by NPM-ALK may be a crucial step for its mitogenic activity and involved in the pathogenesis of anaplastic lymphomas (5).A distinct ALK oncogenic fusion protein involving ALK and echinoderm microtubule-associated protein like 4 (EML4) has been described in the research literature from a non-small cell lung cancer (NSCLC) cell line, with corresponding fusion transcripts present in some cases of lung adenocarcinoma. The short, amino-terminal region of the microtubule-associated protein EML4 is fused to the kinase domain of ALK (6-8).Investigators have identified ALK translocations with other fusion partners, such as TRK-fused gene (TFG) and KIF5B, which have also been associated with NSCLC (6,7). In particular, the EML4-ALK fusion protein has been found in 3-7% of NSCLC samples (6-14).
Reactivity Human
Tested applications WB IP
Clonality Monoclonal Antibody
Calculated MW 80 kDa (NPM-ALK) 220 kDa (ALK)
Recommended Dilutions
FC 1:50
Immunogen A recombinant protein corresponding to residues in the carboxy terminus of human ALK
Storage Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Synonym CD246, Ki-1, TFG/ALK
This product is for research use only, not for diagnostic or therapeutic use!

backtop
QQ
service center
Contact Us