SQSTM1 Rabbit Monoclonal Antibody (Alexa Fluor(R) 488 Conjugate)
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ARM8878-21C 50ul 1500.00 In Stock
ARM8878-21C 100ul 2000.00 In Stock
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Source: Rabbit Gene Id: 8878
Isotype: IgG Swiss Prot: Q13501
purity: Affinity purification
Background:
Sequestosome 1 (SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling, oxidative stress and autophagy (1-4). It was first identified as a protein that binds to the SH2 domain of p56Lck (5) and independently found to interact with PKCζ (6,7). SQSTM1 was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome (8). Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-κB pathway (9). Protein aggregates formed by SQSTM1 can be degraded by the autophagosome (4,10,11). SQSTM1 binds autophagosomal membrane protein LC3/Atg8, bringing SQSTM1-containing protein aggregates to the autophagosome (12). Lysosomal degradation of autophagosomes leads to a decrease in SQSTM1 levels during autophagy; conversely, autophagy inhibitors stabilize SQSTM1 levels. Studies have demonstrated a link between SQSTM1 and oxidative stress. SQSTM1 interacts with KEAP1, which is a cytoplasmic inhibitor of NRF2, a key transcription factor involved in cellular responses to oxidative stress (3). Thus, accumulation of SQSTM1 can lead to an increase in NRF2 activity.
Reactivity Human
Tested applications IF
Clonality Monoclonal Antibody
Calculated MW 62 kDa
Recommended Dilutions
IF 1:50
Immunogen A synthetic peptide corresponding to residues near the carboxy terminus of human SQSTM1/p62 protein
Storage Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Synonym SQSTM1, A170, OSIL, PDB3, ZIP3, p60, p62, p62B
This product is for research use only, not for diagnostic or therapeutic use!

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