Phospho-ULK1-Ser555 Rabbit Monoclonal Antibody
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ARM8408P-21 50ul 1500.00 In Stock
ARM8408P-21 100ul 2000.00 In Stock
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Source: Rabbit Gene Id: 8408
Isotype: IgG Swiss Prot: O75385
purity: Affinity purification
Background:
Two related serine/threonine kinases, UNC-51-like kinase 1 and 2 (ULK1, ULK2), were discovered as mammalian homologs of the C. elegans gene UNC-51 in which mutants exhibited abnormal axonal extension and growth (1-4). Both proteins are widely expressed and contain an amino-terminal kinase domain followed by a central proline/serine rich domain and a highly conserved carboxy-terminal domain. The roles of ULK1 and ULK2 in axon growth have been linked to studies showing that the kinases are localized to neuronal growth cones and are involved in endocytosis of critical growth factors, such as NGF (5). Yeast two-hybrid studies found ULK1/2 associated with modulators of the endocytic pathway, SynGAP and syntenin (6). Structural similarity of ULK1/2 has also been recognized with the yeast autophagy protein Atg1/Apg1 (7). Knockdown experiments using siRNA demonstrated that ULK1 is essential for autophagy (8), a catabolic process for the degradation of bulk cytoplasmic contents (9,10). It appears that Atg1/ULK1 can act as a convergence point for multiple signals that control autophagy (11), and can bind to several autophagy-related (Atg) proteins, regulating phosphorylation states and protein trafficking (12-16).
Reactivity Human, Mouse
Tested applications WB IP
Clonality Monoclonal Antibody
Calculated MW 140-150 kDa
Recommended Dilutions
WB 1:1000
Immunogen A synthetic phosphopeptide corresponding to residues surrounding Ser555 of mouse ULK1 protein (equivalent to Ser556 of human ULK1)
Storage Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Synonym ATG1, FLJ38455, UNC51, Unc51.1
This product is for research use only, not for diagnostic or therapeutic use!

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