| No | Size | Price | Availability | |
| ARM595P-21 | 50ul | 1500.00 | In Stock |  |
| ARM595P-21 | 100ul | 2000.00 | In Stock | |
| Source: | Rabbit | Gene Id: | 595 |
| Isotype: | IgG | Swiss Prot: | P24385 |
| purity: | Affinity purification |
| Background: |
| Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase (2). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation (4).Aberrant expression of cyclin D1 is associated with many forms of cancer, including B cell lymphomas. Gene translocation or amplification of the cyclin D1 gene can directly contribute to oncogenesis (2). Cyclin D1 also plays a critical role in mammary tissue maturation (5). Phosphorylation of cyclin D1 at Thr286 by glycogen synthase kinase 3β (4) or through the Ras/Raf/MEK/MAPK pathway (6) enhances its ubiquitination and proteasomal degradation. |
| Reactivity | Human, Monkey |
| Tested applications | WB IP IF FC |
| Clonality | Monoclonal Antibody |
| Calculated MW | 36 kDa |
| Recommended Dilutions |
WB 1:1000
IF 1:2000
FC 1:1600
|
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Thr286 of cyclin D1 |
| Storage | Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles. |
| Synonym | BCL1, D11S287E, PRAD1, U21B31 |
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