Source: | Rabbit | Gene Id: | / |
Isotype: | / | Swiss Prot: | / |
purity: | Affinity purification |
Background: |
Some signaling molecules can be regulated by phosphorylation at a specific threonine followed by arginine or lysine at the +2 position. For example, conventional PKC isozymes phosphorylate substrates containing serine or threonine with Arg or Lys at the -3, -2 and +2 positions (1-2). c-Raf, a mitogen-activated protein kinase and the main effector recruited by GTP-bound Ras, is phosphorylated at Thr481 and Thr491 followed by Lys at the +2 position (3). Phosphorylation of these sites is important for enzyme activities. |
Reactivity | Human, Monkey, Mouse, Rat, All |
Tested applications | WB IHC |
Clonality | Monoclonal Antibody |
Calculated MW | / |
Recommended Dilutions |
WB 1:1000
IHC 1:800
|
Immunogen | A synthetic peptide containing the phospho-Thr-X-Arg motif |
Storage | Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles. |
Synonym | / |
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