p44/42 MAPK (Erk1/2) Mouse Monoclonal Antibody
No Size Price Availability  
AMMF009-61 50ul 1500.00 In Stock
AMMF009-61 100ul 2000.00 In Stock
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Source: Mouse Gene Id: 5594
Isotype: IgG1 Swiss Prot: P27361/P28482
purity: Affinity chromatography
Background:
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines and is an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors such as U0126 and PD98059.
Reactivity Human, Mouse
Tested applications WB IHC ELISA FC
Clonality Monoclonal Antibody
Calculated MW 42 kDa
Recommended Dilutions
WB 1:500-1:2000
IHC 1:200-1:1000
FC 1:200-1:400
Immunogen A purified recombinant fragment of human p44/42 MAPK (Erk1/2) expressed in E. Coli
Storage Store at -20°C or -80°C in PBS with 0.02% sodium azide and 50% glycerol. Avoid freeze/thaw cycles.
Synonym ERK, p38, p40, p41, ERK2, ERT1, MAPK2, PRKM1, PRKM2, P42MAPK, p41mapk, MAPK1
This product is for research use only, not for diagnostic or therapeutic use!

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