NF-κB p65 Mouse Monoclonal Antibody
No Size Price Availability  
AMM5970-61 50ul 1500.00 In Stock
AMM5970-61 100ul 2000.00 In Stock
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Source: Mouse Gene Id: 5970
Isotype: IgG1 Swiss Prot: Q04206
purity: Affinity chromatography
Background:
Transcription factors of the nuclear factor κ B (NF-κB)/Rel family is a ubiquitously expressed transcription factor that regulates many cytokine and Ig genes. It is involved in immune, inflammatory, viral, and acute phase responses. There are five family members in mammals: RelA (p65), c-Rel, RelB, NF-κB1 (p105/p50) and NF-κB2 (p100/p52). The most studied NF-κB complex consists of the p50 and p65 subunits, both containing a 300 amino acid region with homology to the Rel proto-oncogene product. The p50 subunit binds DNA, whereas the p65 subunit is responsible for the interaction of NF-κB with its inhibitor, IκB. In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to IκB. This complex prevents nuclear translocation and activity of NF-κB. In response to stimuli such as cytokines, LPS, and viral infections, IκB is phosphorylated at critical residues. This phosphorylation induces dissociation of the IκB/NF-κB complex, allowing the free heterodimeric NF-κB to form a heterotetramer that translocates to the nucleus. In the nucleus, it binds to the κB site within promoters and enhancers and functions as a transcriptional activator.
Reactivity Human, Mouse
Tested applications WB ELISA
Clonality Monoclonal Antibody
Calculated MW 65 kDa
Recommended Dilutions
WB 1:500-1:2000
Immunogen A purified recombinant fragment of human NF-κB p65 expressed in E. Coli
Storage Store at -20°C or -80°C in PBS with 0.02% sodium azide and 50% glycerol. Avoid freeze/thaw cycles.
Synonym NFkappaB p65, p65, NFKB3, RELA
This product is for research use only, not for diagnostic or therapeutic use!

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