| No | Size | Price | Availability | |
| ARPF043-21 | 50ul | 1500.00 | In Stock |  |
| ARPF043-21 | 100ul | 2000.00 | In Stock | |
| Source: | Rabbit | Gene Id: | 58/59/60/70/71/72 |
| Isotype: | / | Swiss Prot: | P68133/P62736/P60709/P68032/P63261/P63267 |
| purity: | Affinity purification |
| Background: |
| Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The ARP2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). Research studies have shown that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis (6). |
| Reactivity | Human, Monkey, Mouse, Rat, Zebrafish |
| Tested applications | WB IHC |
| Clonality | Polyclonal Antibody |
| Calculated MW | 45 kDa |
| Recommended Dilutions |
WB 1:1000
IHC 1:50
|
| Immunogen | A synthetic peptide corresponding to residues surrounding Asp244 of human beta-actin |
| Storage | Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles. |
| Synonym | / |
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