| No | Size | Price | Availability | |
| ARP1028P-21 | 50ul | 1500.00 | In Stock |  |
| ARP1028P-21 | 100ul | 2000.00 | In Stock | |
| Source: | Rabbit | Gene Id: | 1028 |
| Isotype: | / | Swiss Prot: | P49918 |
| purity: | Affinity purification |
| Background: |
| p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin-2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4).Levels of p57 Kip2 are controlled by ubiquitination/degradation via the Skp1/Cul1/F-box-type E3 ubiquitin ligase complex SCF-Skp2, and this effect is dependent on Thr310 (5). A similar threonine phosphorylation site in p27 Kip1, Thr187, has also been shown to regulate protein stability (6). |
| Reactivity | Human |
| Tested applications | WB IF |
| Clonality | Polyclonal Antibody |
| Calculated MW | 57 kDa |
| Recommended Dilutions |
WB 1:1000
IF 1:100
|
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Thr310 of human p57 Kip2 |
| Storage | Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles. |
| Synonym | BWCR, BWS, KIP2, WBS, p57 |
沪ICP备15003525号-1 Copyright ©, 2013-2022, Yihyson All Rights Reserved.
