| No | Size | Price | Availability | |
| ARP5347P-11 | 50ul | 1500.00 | In Stock |  |
| ARP5347P-11 | 100ul | 2000.00 | In Stock | |
| Source: | Rabbit | Gene Id: | 5347 |
| Isotype: | / | Swiss Prot: | P53350 |
| purity: | Affinity purification |
| Background: |
| At least four distinct polo-like kinases exist in mammalian cells: PLK1, PLK2, PLK3, and PLK4/SAK (1). PLK1 apparently plays many roles during mitosis, particularly in regulating mitotic entry and exit. The mitosis promoting factor (MPF), cdc2/cyclin B1, is activated by dephosphorylation of cdc2 (Thr14/Tyr15) by cdc25C. PLK1 phosphorylates cdc25C at Ser198 and cyclin B1 at Ser133 causing translocation of these proteins from the cytoplasm to the nucleus (2-5). PLK1 phosphorylation of Myt1 at Ser426 and Thr495 has been proposed to inactivate Myt1, one of the kinases known to phosphorylate cdc2 at Thr14/Tyr15 (6). Polo-like kinases also phosphorylate the cohesin subunit SCC1, causing cohesin displacement from chromosome arms that allow for proper cohesin localization to centromeres (7). Mitotic exit requires activation of the anaphase promoting complex (APC) (8), a ubiquitin ligase responsible for removal of cohesin at centromeres, and degradation of securin, cyclin A, cyclin B1, Aurora A, and cdc20 (9). PLK1 phosphorylation of the APC subunits Apc1, cdc16, and cdc27 has been demonstrated in vitro and has been proposed as a mechanism by which mitotic exit is regulated (10,11). |
| Reactivity | Chiken, Chimpanzee, Xenopus, Dog, Flies, Human, Mouse, Pig, Rat, Zebrafish |
| Tested applications | WB IHC ELISA |
| Clonality | Polyclonal Antibody |
| Calculated MW | 62 kDa |
| Recommended Dilutions |
WB 1:200-1:2000
IHC 1:200-1:1000
|
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding T210 of human PLK1 |
| Storage | Store at 4°C. For long term storage store at -20°C in 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide).Avoid freeze/thaw cycles. |
| Synonym | PLK, STPK13 |
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