CDK5 Rabbit Polyclonal Antibody
No Size Price Availability  
ARP1020-01 50ul 1500.00 In Stock
ARP1020-01 100ul 2000.00 In Stock
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Source: Rabbit Gene Id: 1020
Isotype: IgG Swiss Prot: Q00535
purity: Affinity purification
Background:
Cyclin-dependent kinases (CDKs) are serine/threonine kinases that are activated by cyclins and govern eukaryotic cell cycle progression. While CDK5 shares high sequence homology with its family members, it is thought mainly to function in postmitotic neurons to regulate the cytoarchitecture of these cells. Analogous to cyclins, the regulatory subunits p35 and p39 associate with and activate CDK5 despite the lack of sequence homology. CDK5 is ubiquitously expressed, with high levels of kinase activity detected primarily in the nervous system due to the narrow expression pattern of p35 and p39 in post-mitotic neurons. A large number of CDK5 substrates have been identified although no substrates have been specifically attributed to p35 or p39. Substrates of CDK5 include p35, PAK1, Src, β-catenin, tau, neurofilament-H, neurofilament-M, synapsin-1, APP, DARPP32, PP1-inhibitor, and Rb. p35 is rapidly degraded (T1/2 <20 min) by the ubiquitin-proteasome pathway. However, p35 stability increases as CDK5 kinase activity decreases, likely as a result of decreased phosphorylation of p35 at Thr138 by CDK5. Proteolytic cleavage of p35 by calpain produces p25 upon neurotoxic insult, resulting in prolonged activation of CDK5 by p25. Research studies have shown accumulation of p25 in neurodegenerative diseases, such as Alzheimer's disease and amyotrophic lateral sclerosis (ALS).
Reactivity Human, Mouse, Rat
Tested applications WB IHC
Clonality Polyclonal Antibody
Calculated MW 33 kDa
Recommended Dilutions
WB 1:500-1:2000
IHC 1:50-1:200
Immunogen A recombinant protein of human CDK5
Storage Store at -20°C or -80°C in PBS with 0.02% sodium azide and 50% glycerol. Avoid freeze/thaw cycles.
Synonym PSSALRE
This product is for research use only, not for diagnostic or therapeutic use!

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