| No | Size | Price | Availability | |
| ARP7414-01 | 50ul | 1500.00 | In Stock |  |
| ARP7414-01 | 100ul | 2000.00 | In Stock | |
| Source: | Rabbit | Gene Id: | 7414 |
| Isotype: | IgG | Swiss Prot: | P18206 |
| purity: | Affinity purification |
| Background: |
| Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1-4). Three structural vinculin domains include an amino-terminal head, a short, flexible proline-rich region and a carboxy-terminal tail (1). In the inactive state, the head and tail domains of vinculin interact to form a closed confirmation. The open and active form of vinculin translocates to focal adhesions where it is thought to be involved in anchoring F-actin to the membrane and regulation of cell migration (2). Phospholipid binding to the tail domain and subsequent phosphorylation of vinculin at Ser1033 and Ser1045 by PKC-α and Tyr100 and Tyr1065 by Src kinases weakens the head-tail interaction (5,6). This change in vinculin allows the binding of a number of other proteins, including talin, α-actinin and paxillin, which disrupts the head-tail interaction and initiates the conformational change from the inactive to active state (2,4). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (7,8). This is supported by a recently demonstrated relationship between decreased vinculin expression and increased carcinogenesis and metastasis in colorectal carcinoma (9). |
| Reactivity | Human, Mouse, Rat |
| Tested applications | WB IHC |
| Clonality | Polyclonal Antibody |
| Calculated MW | 124 kDa |
| Recommended Dilutions |
WB 1:500-1:2000
IHC 1:50-1:200
|
| Immunogen | A recombinant protein of human VCL |
| Storage | Store at -20°C or -80°C in PBS with 0.02% sodium azide and 50% glycerol. Avoid freeze/thaw cycles. |
| Synonym | MV, MVCL, CMD1W, CMH15 |
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