Source: | Rabbit | Gene Id: | 28964 |
Isotype: | IgG | Swiss Prot: | |
purity: | Purified by Protein A. |
Background: |
Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist (1,2). G protein-coupled receptor kinases (GRKs) are activated by activated G protein-coupled receptors, and they function to phosphorylate and inactivate cell surface receptors in the heterotrimeric G protein signaling cascade (3,4). GIT1 (for GRK-interactor 1) and GIT2 are GTPase-activating proteins (GAP) for members of the ADP ribosylation factor (ARF) family of small GTP-binding proteins, which are involved in vesicular trafficking (5,6). GIT1 overexpression results in reduced internalization and resensitization of b2-adrenergic receptor, thus reducing b2-adrenergic receptor signaling (5). |
Reactivity | Human, Mouse, Rat |
Tested applications | WB IHC IF |
Clonality | Polyclonal Antibody |
Calculated MW | / |
Recommended Dilutions |
WB 1:100-1:1000
IHC 1:100-1:500
IF 1:50-1:200
|
Immunogen | KLH conjugated synthetic phosphopeptide derived from human GIT1 around the phosphorylation site of Tyr545 |
Storage | Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at -20℃ for 12 months |
Synonym | GIT1 phospho Tyr545, p-GIT1 Tyr545, ARF GAP GIT1, ARF GTPase activating protein GIT1, CAT 1, Cat1, Cool associated and tyrosine phosphorylated protein 1, G protein coupled receptor kinase interactor 1, GRK interacting protein 1. |
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