MonoMethyl-Histone H3-R2 Rabbit Polyclonal Antibody
No Size Price Availability  
ARP8290MM-06 50ul 1500.00 In Stock
ARP8290MM-06 100ul 2000.00 In Stock
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Source: Rabbit Gene Id: 8290
Isotype: IgG Swiss Prot: Q16695
purity: Affinity purification
Background:
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
Reactivity Human, Mouse, Rat, Other, (Wide, Ratange)
Tested applications WB IHC IF CHIP
Clonality Polyclonal Antibody
Calculated MW 15kDa
Recommended Dilutions
WB 1:500-1:2000
IHC 1:50-1:200
IF 1:50-1:200
ChIP 1:50-1:200
Immunogen A synthetic methylated peptide corresponding to residues surrounding R2 of human histone H3
Storage Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Synonym HIST1H3J, H3/j ;H3FJ , Histone H3.1, Histone H3/a, Histone H3/b;Histone H3/c, Histone H3/d, Histone H3/f;Histone H3/h , Histone H3/I;Histone H3/j , Histone H3/k, Histone H3/l, HIST3H3
This product is for research use only, not for diagnostic or therapeutic use!

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