| No | Size | Price | Availability | |
| ARPF057-51 | 50ul | 1500.00 | In Stock |  |
| ARPF057-51 | 100ul | 2000.00 | In Stock | |
| Source: | Rabbit | Gene Id: | 81631/84557 |
| Isotype: | IgG | Swiss Prot: | Q9GZQ8/Q9H492 |
| purity: | Affinity purification |
| Background: |
| Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection, and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4) and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy (11). |
| Reactivity | Chicken, Cow, Dog, Horse, Human, Mouse, Pig, Rat |
| Tested applications | WB ELISA IP IF |
| Clonality | Polyclonal Antibody |
| Calculated MW | 15 kDa |
| Recommended Dilutions |
WB 1:100-1:1000
IF 1:50-1:500
|
| Immunogen | A synthetic peptide corresponding to amino acids 58-104 mapping within an internal region of LC3A |
| Storage | Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles. |
| Synonym | / |
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