MET Rabbit Polyclonal Antibody
No Size Price Availability  
ARP4233-11 50ul 1500.00 In Stock
ARP4233-11 100ul 2000.00 In Stock
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Source: Rabbit Gene Id: 4233
Isotype: IgG Swiss Prot: P08581
purity: Affinity purification
Background:
Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl, and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation at Tyr1234/1235 in the Met kinase domain is critical for kinase activation. Phosphorylation at Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Research studies have shown that altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon, and breast. Thus, investigators have concluded that Met is an attractive potential cancer therapeutic and diagnostic target (6,7).
Reactivity Human, Mouse, Rat
Tested applications WB
Clonality Polyclonal Antibody
Calculated MW 156 kDa
Recommended Dilutions
WB 1:500-1:1000
Immunogen A synthetic peptide corresponding to residues surrounding Y1234 of human MET
Storage Store at -20°C or -80°C in PBS with 0.02% sodium azide and 50% glycerol. Avoid freeze/thaw cycles.
Synonym MET, AUTS9, HGFR, RCCP2, c-Met
This product is for research use only, not for diagnostic or therapeutic use!

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