| No | Size | Price | Availability | |
| ARP995P-22 | 50ul | 1500.00 | In Stock |  |
| ARP995P-22 | 100ul | 2000.00 | In Stock | |
| Source: | Rabbit | Gene Id: | 995 |
| Isotype: | / | Swiss Prot: | P30307 |
| purity: | Affinity purification |
| Background: |
| Cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm and thereby preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5).During prophase, polo-like kinase 1 (PLK1) phosphorylates cdc25C at Ser198, causing translocation from the cytoplasm to the nucleus, where cdc25C can interact with cdc2/cyclin B to allow for progression through the remaining stages of mitosis (6). |
| Reactivity | Human |
| Tested applications | WB FC |
| Clonality | Polyclonal Antibody |
| Calculated MW | 75 kDa |
| Recommended Dilutions |
WB 1:1000
FC 1:25
|
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Ser198 of human cdc25C |
| Storage | Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles. |
| Synonym | CDC25, PPP1R60 |
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