Source: | Rabbit | Gene Id: | 2931/2932 |
Isotype: | / | Swiss Prot: | P49840/P49841 |
purity: | Affinity purification |
Background: |
Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). |
Reactivity | Human, Monkey, Mouse, Rat, Zebrafish |
Tested applications | WB IHC |
Clonality | Polyclonal Antibody |
Calculated MW | 46 kDa GSK-3beta. 51 kDa GSK-3alpha. |
Recommended Dilutions |
WB 1:1000
IHC 1:50
|
Immunogen | A synthetic phosphopeptide corresponding to the sequence of human GSK-3 alpha |
Storage | Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles. |
Synonym | / |
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