Source: | Rabbit | Gene Id: | 2777477(Phage) |
Isotype: | / | Swiss Prot: | P06956 |
purity: | Affinity purification |
Background: |
Cre recombinase is a bacteriophage-P1 enzyme required for maintenance of the phage genome as a monomeric plasmid in the lysogenic state (1,2). This enzyme mediates a site-specific recombination between two 34 base pair loxP sites. This reaction can be carried out in vitro, indicating that it does not require accessory factors (3). The Cre/Lox system has been used for a number of in vitro and in vivo applications including targeted gene deletions (4) and gene-specific humanized animal models (5). Resolution of the crystal structure of the Cre-Lox complex revealed that two Cre molecules interact with a single Lox site (6). |
Reactivity | All |
Tested applications | WB |
Clonality | Polyclonal Antibody |
Calculated MW | 38 kDa |
Recommended Dilutions |
WB 1:1000
|
Immunogen | A synthetic peptide corresponding to residues surrounding Asp141 of Cre recombinase protein |
Storage | Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles. |
Synonym | P1_gp003 |
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