BECN1 Rabbit Polyclonal Antibody
No Size Price Availability  
ARP8678-31 50ul 1500.00 In Stock
ARP8678-31 100ul 2000.00 In Stock
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Source: Rabbit Gene Id: 8678
Isotype: IgG Swiss Prot: Q14457
purity: Affinity purification
Background:
Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of proteins activated in response to nutrient deprivation and in neurodegenerative conditions (1). One of the proteins critical to this process is Beclin-1, the mammalian orthologue of the yeast autophagy protein Apg6/Vps30 (2). Beclin-1 can complement defects in yeast autophagy caused by loss of Apg6 and can also stimulate autophagy when overexpressed in mammalian cells (3). Mammalian Beclin-1 was originally isolated in a yeast two-hybrid screen for Bcl-2 interacting proteins and has been shown to interact with Bcl-2 and Bcl-xL but not with Bax or Bak (4). While Beclin-1 is generally ubiquitously expressed, it is monoallelically deleted in 40-75% of sporadic human breast and ovarian cancers (5). It is localized within cytoplasmic structures including the mitochondria, although overexpression of Beclin-1 reveals some nuclear staining and CRM1-dependent nuclear export (6). Beclin-1 -/- mice die early in embryogenesis and Beclin-1 -/+ mice have a high incidence of spontaneous tumors. Stem cells from the null mice demonstrate an altered autophagic response although responses to apoptosis appeared normal (7). Overexpression of Beclin-1 in virally infected neurons in vivo resulted in significant protection against Sindbis virus-induced disease and neuronal apoptosis (4).
Reactivity Human, Mouse, Rat
Tested applications WB
Clonality Polyclonal Antibody
Calculated MW 52 kDa
Recommended Dilutions
WB 1:100-1:400
Immunogen A recombinant protein of human Beclin-1
Storage Store at -20°C or -80°C in PBS with 0.02% sodium azide and 50% glycerol. Avoid freeze/thaw cycles.
Synonym BECN1, ATG6, VPS30, beclin1
This product is for research use only, not for diagnostic or therapeutic use!

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