| No | Size | Price | Availability | |
| ARPF060-21 | 50ul | 1500.00 | In Stock |  |
| ARPF060-21 | 100ul | 2000.00 | In Stock | |
| Source: | Rabbit | Gene Id: | 997/54926 |
| Isotype: | / | Swiss Prot: | P49427/Q712K3 |
| purity: | Affinity purification |
| Background: |
| Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3). Combinatorial interactions of different E2 and E3 proteins result in substrate specificity (4). Recent data suggest that activated E2 associates transiently with E3, and that the dissociation is a critical step for ubiqitination (5). UBC3, the mammalian orthologue of yeast Cdc34, and UBC3B, a UBC3 family member, are E2 ubiquitin-carrier proteins. These proteins contain a conserved core domain containing a cysteine residue, which forms the thioester bond with ubiquitin (6). UBC3 in concert with the SCFSkp2 (Skp1, Cullin and F-box protein/Skp2) complex mediates cell cycle progression from G1 to S phase by targeting the CDK inhibitor p27 for proteolysis (7). UBC3B in concert with the SCFb-Trcp (Skp1, Cullin and F-box protein/b-Trcp) complex mediates degradation of b-catenin (6). |
| Reactivity | Human, Mouse, Rat |
| Tested applications | WB FC |
| Clonality | Polyclonal Antibody |
| Calculated MW | 32 kDa |
| Recommended Dilutions |
WB 1:1000
FC 1:25
|
| Immunogen | A synthetic peptide corresponding to the sequence of human UBC3 |
| Storage | Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles. |
| Synonym | / |
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