| No | Size | Price | Availability | |
| ARP10398P-22 | 50ul | 1500.00 | In Stock |  |
| ARP10398P-22 | 100ul | 2000.00 | In Stock | |
| Source: | Rabbit | Gene Id: | 10398 |
| Isotype: | / | Swiss Prot: | P24844 |
| purity: | Affinity purification |
| Background: |
| Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC), RLC, or LC20, has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3). Phosphorylation of smooth muscle MLC2 at Ser1/Ser2 and Ser9 by PKC and cdc2 has been reported to inhibit myosin ATPase activity (4,5). Phosphorylation by cdc2 controls the timing of cytokinesis (5). Transgenic mice lacking phosphorylation sites on the cardiac muscle isoform show morphological and functional abnormalities (6). |
| Reactivity | Human, Mouse |
| Tested applications | WB |
| Clonality | Polyclonal Antibody |
| Calculated MW | 18 kDa |
| Recommended Dilutions |
WB 1:1000
|
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Thr18/Ser19 of human myosin light chain 2 |
| Storage | Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles. |
| Synonym | LC20, MLC2, MRLC1, MYRL2, MLC-2C |
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