| No | Size | Price | Availability | |
| ARPF051P-21 | 50ul | 1500.00 | In Stock |  |
| ARPF051P-21 | 100ul | 2000.00 | In Stock | |
| Source: | Rabbit | Gene Id: | 6850/7535 |
| Isotype: | / | Swiss Prot: | P43405/P43403 |
| purity: | Affinity purification |
| Background: |
| The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).Phosphorylation of Tyr319 is required for the assembly of a Zap-70-containing signaling complex that leads to the activation of the PLC-gamma1-dependent and Ras-dependent signaling cascades in antigen-stimulated T cells (5,6). The orthologous Tyr352 residue in Syk is also involved in the association with PLC-gamma1 (9). |
| Reactivity | Human |
| Tested applications | WB IP IF FC |
| Clonality | Polyclonal Antibody |
| Calculated MW | 70 kDa Zap-70, 72 kDa Syk |
| Recommended Dilutions |
WB 1:1000
IF 1:100
FC 1:50
|
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Tyr319 of human Zap-70 |
| Storage | Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles. |
| Synonym | / |
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