| No | Size | Price | Availability | |
| ARP9101-12 | 50ul | 1500.00 | In Stock |  |
| ARP9101-12 | 100ul | 2000.00 | In Stock | |
| Source: | Rabbit | Gene Id: | 9101 |
| Isotype: | / | Swiss Prot: | P40818 |
| purity: | Protein G purification |
| Background: |
| Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action (1,2). Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes. The deubiquitinating enzyme ubiquitin-specific protease 8 (USP8/UBPy) is a cysteine protease belonging to the USP/UBP subfamily. Research studies have shown that USP8 is an essential growth-regulated enzyme indespensible for cell proliferation and survival (3,4). Indeed, conditional knock-out of murine USP8 was shown to promote a dramatic loss in expression of receptor tyrosine kinases, including EGFR, ErbB3, and c-Met (4). In agreement with these findings, USP8 inactivation leads to enhanced ubiquitination of ligand-activated EGFR (5,6). Furthermore, phosphorylation of USP8 at Ser680 results in its binding of 14-3-3, catalytic inactivation, and reduced EGFR deubiquitination (7). It appears as though USP8, in conjunction with components of the ESCRT-0 complex, plays an integral role in the early endosomal sorting machinery that functions to protect EGFR from lysosomal degradation (8,9). |
| Reactivity | Human |
| Tested applications | WB IHC ELISA |
| Clonality | Polyclonal Antibody |
| Calculated MW | 140 kDa |
| Recommended Dilutions |
WB 1:100-1:500
IHC 1:50-1:100
|
| Immunogen | A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human USP8 |
| Storage | Store at 4°C. For long term storage store at -20°C in PBS (0.09% sodium azide).Avoid freeze/thaw cycles. |
| Synonym | FLJ34456, HumORF8, KIAA0055, MGC129718, UBPY |
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