| No | Size | Price | Availability | |
| ARP9833-12 | 50ul | 1500.00 | In Stock |  |
| ARP9833-12 | 100ul | 2000.00 | In Stock | |
| Source: | Rabbit | Gene Id: | 9833 |
| Isotype: | / | Swiss Prot: | Q14680 |
| purity: | Ammonium sulfate precipitation |
| Background: |
| MELK (Maternal Embryonic Leucine zipper Kinase, MPK38, KIAA0175) is a member of the Snf1/AMPK related kinase family. It is implicated in stem cell renewal, cell cycle progression and pre-m-RNA splicing (1,2,3). MELK is also a marker for self-renewing multipotent neural progenators, and may function in embryonic and postnatal forebrain development (4). While other members of this kinase family are activated by LKB1 and CAMKII mediated phosphorylation of the T-loop, MELK is not (5,6,7). Regulation of activation appears complex since MELK overexpressed in mammalian cells is inactive (7). Some evidence suggests that activation occurs through autophosphorylation of Thr167 and Ser171, although a number of additional autophosphorylation sites have been suggested (8). Recently, phosphorylations of Thr449, Thr451 and Thr481 have been specifically detected during mitosis, and are thought to occur via MPF and MAPK pathways (9). MELK has broad substrate specificity in vitro: substrates include ZPR9 (10), NIPP1 (11) and cdc25B (12), although the significance of MELK mediated phosphorylation of these proteins is unclear. |
| Reactivity | Human |
| Tested applications | WB IHC IF |
| Clonality | Polyclonal Antibody |
| Calculated MW | 74 kDa |
| Recommended Dilutions |
WB 1:500-1:1000
IHC 1:50-1:200
IF 1:50-1:200
|
| Immunogen | A synthetic peptide corresponding to MELK |
| Storage | Store at 4°C. For long term storage store at -20°C in PBS, pH 7.2 (0.05% sodium azide).Avoid freeze/thaw cycles. |
| Synonym | HPK38, KIAA0175 |
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