| No | Size | Price | Availability | |
| ARP5970-64 | 50ul | 1500.00 | In Stock |  |
| ARP5970-64 | 100ul | 2000.00 | In Stock | |
| Source: | Rabbit | Gene Id: | 5970 |
| Isotype: | IgG | Swiss Prot: | Q04206 |
| purity: | Affinity purification |
| Background: |
| Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11). NF-κB assembly with IκB, as well as its DNA binding and transcriptional activity, are regulated by p300/CBP acetytransferases that principally target Lys218, Lys221 and Lys310 (12-14). This process is reciprocally regulated by histone deacetylases (HDACs); several HDAC inhibitors have been shown to activate NF-κB (12-14). |
| Reactivity | Human, Mouse, Rat |
| Tested applications | WB IHC |
| Clonality | Polyclonal Antibody |
| Calculated MW | 65 kDa |
| Recommended Dilutions |
WB 1:500-1:2000
IHC 1:50-1:200
|
| Immunogen | A recombinant protein of human p65 |
| Storage | Store at -20°C or -80°C in PBS with 0.02% sodium azide and 50% glycerol. Avoid freeze/thaw cycles. |
| Synonym | RELA,MGC131774,NFKB3,p65 |
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