Source: | Rabbit | Gene Id: | / |
Isotype: | IgG | Swiss Prot: | / |
purity: | Affinity purification |
Background: |
Plasmid vectors for the expression of coding regions of eukaryotic genes in E.coli are in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. Forinstance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase(GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression bothin plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. Coli. |
Reactivity | / |
Tested applications | WB IP IF |
Clonality | Polyclonal Antibody |
Calculated MW | / |
Recommended Dilutions |
WB 1:1000-1:2000
IF 1:500
|
Immunogen | A recombinant protein of human GST-Tag |
Storage | Store at -20°C or -80°C in PBS with 0.02% sodium azide and 50% glycerol. Avoid freeze/thaw cycles. |
Synonym | / |
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