SETD8 Rabbit Polyclonal Antibody
No Size Price Availability  
ARP387893-01 50ul 1500.00 In Stock
ARP387893-01 100ul 2000.00 In Stock
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Source: Rabbit Gene Id: 387893
Isotype: IgG Swiss Prot: Q9NQR1
purity: Affinity purification
Background:
SET domain-containing lysine methyltransferase 8 (SET8), also known as PR/SET domain-containing protein 7 (PR/SET7), is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins. SET8 is a single-subunit enzyme that mono-methylates histone H4 on Lys20, preferably on nucleosomal substrates. SET8 protein levels and Histone H4 Lys20 methylation are cell cycle regulated, both increasing in S phase and peaking at G2/M phase. SET8 interacts with the PCNA protein, associates with sites of active DNA synthesis, and is required for DNA replication and genome stability during S phase. Inhibition of SET8 using shRNA or siRNA results in arrest of replication forks, induction of double-stranded DNA breaks, and a Chk1-mediated cell-cycle arrest in S and G2/M phases of the cell cycle. Furthermore, SET8 methylates p53 on Lys382, down regulating the pro-apoptotic and checkpoint activation functions of p53. In response to DNA damage, SET8 expression levels decrease, allowing p53 to activate checkpoints and/or apoptosis. Both the methylation of histone H4 Lys20 and p53 appear to be important for the functions of SET8 in S phase.
Reactivity Human, Mouse, Rat
Tested applications WB IHC
Clonality Polyclonal Antibody
Calculated MW 42 kDa
Recommended Dilutions
WB 1:500-1:2000
IHC 1:50-1:200
Immunogen A recombinant protein of human SETD8
Storage Store at -20°C or -80°C in PBS with 0.02% sodium azide and 50% glycerol. Avoid freeze/thaw cycles.
Synonym SET8, KMT5A, SET07, PR-Set7
This product is for research use only, not for diagnostic or therapeutic use!

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