PAK1 Rabbit Polyclonal Antibody
No Size Price Availability  
ARP5058-01 50ul 1500.00 In Stock
ARP5058-01 100ul 2000.00 In Stock
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Source: Rabbit Gene Id: 5058
Isotype: IgG Swiss Prot: Q13153
purity: Affinity purification
Background:
The p21-activated kinase (PAK) family of serine/threonine kinases is engaged in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, control of phagocyte NADPH oxidase, and growth factor-induced neurite outgrowth (1,2). Several mechanisms that induce PAK activity have been reported. Binding of Rac/Cdc42 to the CRIB (or PBD) domain near the amino terminus of PAK causes autophosphorylation and conformational changes in PAK (1). Phosphorylation of PAK1 at Thr423 by PDK induces activation of PAK1 (3). Several autophosphorylation sites have been identified, including Ser199 and Ser204 of PAK1 and Ser192 and Ser197 of PAK2 (4,5). Because the autophosphorylation sites are located in the amino-terminal inhibitory domain, it has been hypothesized that modification in this region prevents the kinase from reverting to an inactive conformation (6). Research indicates that phosphorylation at Ser144 of PAK1 or Ser139 of PAK3 (located in the kinase inhibitory domain) affects kinase activity (7). Phosphorylation at Ser21 of PAK1 or Ser20 of PAK2 regulates binding with the adaptor protein Nck (8). PAK4, PAK5, and PAK6 have lower sequence similarity with PAK1-3 in the amino-terminal regulatory region (9). Phosphorylation at Ser474 of PAK4, a site analogous to Thr423 of PAK1, may play a pivotal role in regulating the activity and function of PAK4 (10).
Reactivity Human, Mouse, Rat
Tested applications WB IHC IP ICC
Clonality Polyclonal Antibody
Calculated MW 62 kDa
Recommended Dilutions
WB 1:500-1:2000
IHC 1:50-1:200
ICC 1:50-1:200
Immunogen A recombinant protein of human PAK1
Storage Store at -20°C or -80°C in PBS with 0.02% sodium azide and 50% glycerol. Avoid freeze/thaw cycles.
Synonym MGC130000, MGC130001, PAKalpha
This product is for research use only, not for diagnostic or therapeutic use!

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