CRK Rabbit Polyclonal Antibody
No Size Price Availability  
ARP1398-11 50ul 1500.00 In Stock
ARP1398-11 100ul 2000.00 In Stock
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Source: Rabbit Gene Id: 1398
Isotype: / Swiss Prot: P46108
purity: Affinity purification
Background:
CrkII, a cellular homologue of v-Crk, belongs to a family of adaptor proteins with an SH2-SH3-SH3 domain structure that transmits signals from tyrosine kinases (1). The primary function of Crk is to recruit cytoplasmic proteins in the vicinity of tyrosine kinases through SH2-phospho-tyrosine interaction. Thus, the output from Crk depends on the SH3-binding proteins, which include the C3G and Sos guanine nucleotide exchange proteins, Abl tyrosine kinase, DOCK180 and some STE20-related kinases. The variety of Crk-binding proteins indicates the pleiotropic function of Crk (2). The two CrkII SH3 domains are separated by a 54 amino acid linker region, which is highly conserved in Xenopus, chicken and mammalian CrkII proteins (3). Tyrosine 221 in this region is phosphorylated by the Abl tyrosine kinase (4), IGF-I receptor (5) and EGF receptor (6). Once Tyr221 is phosphorylated, CrkII undergoes a change in intramolecular folding and SH2-pTyr interaction, which causes rapid dissociation of CrkII from the tyrosine kinase complex (3).
Reactivity Human
Tested applications WB IF PLA
Clonality Polyclonal Antibody
Calculated MW 22.90 kDa
Recommended Dilutions
Immunogen CRK (NP_005197.3, 1 a.a. ~ 204 a.a) full-length human protein
Storage Store at -20°C or lower in PBS(pH 7.4). Avoid freeze/thaw cycles.
Synonym CRKII
This product is for research use only, not for diagnostic or therapeutic use!

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