AMM5599P-21C [Monoclonal Antibody]
Phospho-MAPK8-Thr183/Tyr185 Mouse Monoclonal Antibody (Alexa Fluor(R) 647 Conjugate)
www.yhsbio.com
market@yhsbio.com
support@yhsbio.com
+86-21-54651191
Room 703,Building 6,333# Guiping
Rd.,Xuhui District,Shanghai,China
DATASHEET
Species:   Mouse
Applications:   FC
Immunogen Range:   A synthetic phosphopeptide corresponding to residues around Thr183/Tyr185 of human SAPK/JNK
Clonality:   Monoclonal Antibody
Isotype:   IgG1
GENE ID:   5599
Swiss Prot:   P45983
Synonyms:   JNK, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1
Purification:   Affinity purification
Storage:   Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Background:   The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing, resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5).
Caculated MW:   46 kDa Phospho-JNK1 54 kDa Phospho-JNK2/3
Observed MW:   Refer to Figures
Applications:   FC 1:50
Reacitivity:   Hamster, Human, Mouse, Rat, S. cerevisiae
For research use only. Not intended for diagnostic or therapeutic use!
Additional information