ARM5777P-21
[Monoclonal Antibody]
Phospho-PTPN6-Tyr564 Rabbit Monoclonal Antibody
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DATASHEET
Species: |
Rabbit |
Applications: |
WB |
Immunogen Range: |
A synthetic phosphopeptide corresponding to residues surrounding Tyr564 of human SHP-1 protein |
Clonality: |
Monoclonal Antibody |
Isotype: |
IgG |
GENE ID: |
5777 |
Swiss Prot: |
P29350 |
Synonyms: |
HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1, SHP-1L, SHP1, SH-PTP1 |
Purification: |
Affinity purification |
Storage: |
Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles. |
Background: |
SHP-1 (PTPN6) is a non-receptor protein tyrosine phosphatase that is expressed primarily in hematopoietic cells. The enzyme is composed of two SH2 domains, a tyrosine phosphatase catalytic domain, and a carboxy-terminal regulatory domain (1). SHP-1 removes phosphates from target proteins to downregulate several tyrosine kinase-regulated pathways. In hematopoietic cells, the amino-terminal SH2 domain of SHP-1 binds to tyrosine phosphorylated erythropoietin receptors (EpoR) to negatively regulate hematopoietic growth (2). Overexpression of SHP-1 in epithelial cells results in dephosphorylation of the Ros receptor tyrosine kinase and subsequent downregulation of Ros-dependent cell proliferation and transformation (3). Following ligand binding in myeloid cells, SHP-1 associates with the IL-3R β chain and downregulates IL-3-induced tyrosine phosphorylation and cell proliferation (4). Because SHP-1 downregulates various proliferation pathways, SHP-1 is considered a potential tumor suppressor and angiogenesis regulator (5,6).SHP-1 is a substrate of Src family kinases (7,8) and phosphorylation of Tyr564 is thought to be critical for achieving maximal phosphatase activity (8). In a murine model of chronic myelomonocytic leukemia (CMML), genetic suppression of Tyr564 phosphorylation led to constitutive overactivation of the transcription factor Stat5 and an accelerated onset of CMML-like disease (8). |
Caculated MW: |
68 kDa |
Observed MW: |
Refer to Figures |
Applications: |
WB 1:1000
|
Reacitivity: |
Human, Mouse |
For research use only. Not intended for diagnostic or therapeutic use!