Species: | Rabbit |
Applications: | WB IF FC |
Immunogen Range: | A synthetic peptide corresponding to residues surrounding Ser465/467 of human Smad2 protein |
Clonality: | Polyclonal Antibody |
Isotype: | IgG |
GENE ID: | 4087/4088 |
Swiss Prot: | Q15796/P84022 |
Synonyms: | / |
Purification: | Affinity purification |
Storage: | Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles. |
Background: | Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8). |
Caculated MW: | 52, 60 kDa |
Observed MW: | Refer to Figures |
Applications: |
WB 1:1000 IF 1:200 FC 1:800 |
Reacitivity: | Human, Monkey, Mouse, Rat |