YRP13738-01 [Polyclonal Antibody]
GAL4 Rabbit Polyclonal Antibody
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Species:   Rabbit
Applications:   WB IHC IF
Immunogen Range:   KLH conjugated synthetic peptide derived from Saccharomyces cerevisiae GAL4
Clonality:   Polyclonal Antibody
Isotype:   IgG
GENE ID:  
Swiss Prot:  
Synonyms:   Gal4p, GAL81, Homo sapiens galectin4 mRNA complete cds, Regulatory protein GAL4.
Purification:   Purified by Protein A.
Storage:   Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at -20℃ for 12 months
Background:   The GAL4 protein of Saccharomyces cerevisiae is one of the most thoroughly characterized transcriptional activators. As the N-terminal 147 amino acid residues of GAL4 are sufficient to mediate specific and strong binding to DNA, but are incapable of efficient transcriptional activation, this protein fragment has frequently been used to confer specific DNA binding in experiments examining transcriptional activation functions of heterologous proteins. This approach is facilitated by the finding that higher eukaryotes lack endogenous proteins that enhance transcription from the consensus GAL4-binding site. Fusions between GAL4 (amino acids 1-147) and activating domains from a variety of transcriptional regulatory proteins can activate transcription in yeast, plant, insects and mammalian cells. Fields and coworkers have taken advantage of these findings by the development of a unique “two-hybrid” system using GAL4 fusions in yeast to identify specific protein-protein interactions.
Caculated MW:   /
Observed MW:   Refer to Figures
Applications:   WB 1:100-1:1000
IHC 1:100-1:500
IF 1:50-1:200
Reacitivity:   Yeast (Saccharomyces cerevisiae)
For research use only. Not intended for diagnostic or therapeutic use!
Additional information