ARP5788-01 [Polyclonal Antibody]
PTPRC Rabbit Polyclonal Antibody
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Species:   Rabbit
Applications:   WB
Immunogen Range:   A synthetic peptide of human PTPRC
Clonality:   Polyclonal Antibody
Isotype:   IgG
GENE ID:   5788
Swiss Prot:   P08575
Synonyms:   B220, CD45, CD45R, GP180, LCA, LY5, T200
Purification:   Affinity purification
Storage:   Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Background:   The protein phosphatase (PTP) receptor CD45 is a type I transmembrane protein comprised of a pair of intracellular tyrosine phosphatase domains and a variable extracellular domain generated by alternative splicing (1). The catalytic activity of CD45 is a function of the first phosphatase domain (D1) while the second phosphatase domain (D2) may interact with and stabilize the first domain, or recruit/bind substrates (2,3). CD45 interacts directly with antigen receptor complex proteins or activates Src family kinases involved in the regulation of T- and B-cell antigen receptor signaling (1). Specifically, CD45 dephosphorylates Src-family kinases Lck and Fyn at their conserved negative regulatory carboxy-terminal tyrosine residues and upregulates kinase activity. Conversely, studies indicate that CD45 can also inhibit Lck and Fyn by dephosphorylating their positive regulatory autophosphorylation site. CD45 appears to be both a positive and a negative regulator that conducts signals depending on specific stimuli and cell type (1). Human leukocytes including lymphocytes, eosinophils, monocytes, basophils and neutrophils express CD45, while erythrocytes and platelets are negative for CD45 expression (4).
Caculated MW:   147kDa
Observed MW:   Refer to Figures
Applications:   WB 1:500-1:2000
Reacitivity:   Human, Mouse
For research use only. Not intended for diagnostic or therapeutic use!
Additional information