ARPF057-51 [Polyclonal Antibody]
LC3A/B Rabbit Polyclonal Antibody
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Species:   Rabbit
Applications:   WB ELISA IP IF
Immunogen Range:   A synthetic peptide corresponding to amino acids 58-104 mapping within an internal region of LC3A
Clonality:   Polyclonal Antibody
Isotype:   IgG
GENE ID:   81631/84557
Swiss Prot:   Q9GZQ8/Q9H492
Synonyms:   /
Purification:   Affinity purification
Storage:   Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Background:   Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection, and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4) and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy (11).
Caculated MW:   15 kDa
Observed MW:   Refer to Figures
Applications:   WB 1:100-1:1000
IF 1:50-1:500
Reacitivity:   Chicken, Cow, Dog, Horse, Human, Mouse, Pig, Rat
For research use only. Not intended for diagnostic or therapeutic use!
Additional information