Species: | Rabbit |
Applications: | WB IHC ELISA IF ICC |
Immunogen Range: | A synthetic peptide derived from human FYB/ADAP |
Clonality: | Polyclonal Antibody |
Isotype: | IgG |
GENE ID: | 2533 |
Swiss Prot: | O15117 |
Synonyms: | ADAP, PRO0823, SLAP130, SLAP-130 |
Purification: | Affinity purification |
Storage: | Store at -20°C or -80°C in PBS with 0.02% sodium azide and 50% glycerol. Avoid freeze/thaw cycles. |
Background: | Fyb (Fyn binding protein) and the anchoring proteins SKAP55 (src kinase-associated phosphoprotein) and SKAP55-R (SKAP55-related protein) associate with the tyrosine kinase p59fyn. SKAP55 and SKAP55-R bind to Fyb through their SH3 domains and function as substrates for p59Fyn in resting T cells (1–3). SKAP55 contains an amino-terminal pleckstrin homology domain and a carboxy-terminal SH3 domain binding motif of adjacent arginine and lysine residues followed by tandem tyrosines (i.e. RKxxYxxY). SKAP55-R, similar in overall structure to SKAP55, contains a coiled-coil N-terminal domain. SKAP55 associates with SLAP-130, another component of the Fyn complex, which plays a role in the regulation of signaling events initiated by lymphocyte antigen receptors leading up to T cell activation. The human Fyb gene maps to chromosome 5p13.1 and encodes a 783 amino acid protein. |
Caculated MW: | 85 kDa |
Observed MW: | Refer to Figures |
Applications: |
WB 1:100-1:500 IHC 1:100-1:500 IF 1:100-1:500 ICC 1:100-1:500 |
Reacitivity: | Chicken, Cow, Dog, Horse, Human, Mouse, Pig, Rat, Sheep |