ARP1676-21 [Polyclonal Antibody]
Cleaved DFFA (Asp224) Rabbit Polyclonal Antibody
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Species:   Rabbit
Applications:   WB
Immunogen Range:   A synthetic peptide corresponding to carboxy-terminal residues surrounding Asp224 of human DFF45
Clonality:   Polyclonal Antibody
Isotype:   /
GENE ID:   1676
Swiss Prot:   O00273
Synonyms:   DFF1, ICAD, DFF-45
Purification:   Affinity purification
Storage:   Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Background:   Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF), heterodimer of 40-kDa (DFFB) and 45-kDa (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. DFFA appears to be a 45-kDa protein despite the predicated MW of 37 kDa.
Caculated MW:   12 kDa
Observed MW:   Refer to Figures
Applications:   WB 1:1000
Reacitivity:   Human
For research use only. Not intended for diagnostic or therapeutic use!
Additional information