MRM11545C-21S [Monoclonal Antibody]
Cleaved PARP1 (Asp214) Mouse Monoclonal Antibody (Mouse Specific)
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Species:   Mouse
Applications:   WB
Immunogen Range:   A synthetic PARP peptide corresponding to carboxy-terminal residues surrounding Asp214 in mouse PARP
Clonality:   Monoclonal Antibody
Isotype:   IgG2b
GENE ID:   11545(Mouse)
Swiss Prot:   P11103
Synonyms:   5830444G22Rik, AI893648, ARTD1, Adprp, Adprt1, C80510, PPOL, parp-1, sPARP-1
Purification:   Affinity purification
Storage:   Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Background:   PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)
Caculated MW:   89 kDa
Observed MW:   Refer to Figures
Applications:   WB 1:1000
Reacitivity:   Mouse
For research use only. Not intended for diagnostic or therapeutic use!
Additional information