ARMF009P-25C [Monoclonal Antibody]
Phospho-MAPK1/3-Thr202/Tyr204 Rabbit Monoclonal Antibody (Magnetic Bead Conjugate)
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Species:   Rabbit
Applications:   IP
Immunogen Range:   A synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase
Clonality:   Monoclonal Antibody
Isotype:   IgG
GENE ID:   5594/5595
Swiss Prot:   P27361/P28482
Synonyms:   /
Purification:   Affinity purification
Storage:   Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Background:   Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors, such as U0126 and PD98059.
Caculated MW:   42, 44 kDa
Observed MW:   Refer to Figures
Applications:  
Reacitivity:   Bovine, D. melanogaster, Dog, Hamster, Human, Monkey, Mouse, Pig, Rat, S. cerevisiae, Zebrafish
For research use only. Not intended for diagnostic or therapeutic use!
Additional information