AMMF013P-23C [Monoclonal Antibody]
Phospho-p38 MAPK-Thr180/Tyr182 Mouse Monoclonal Antibody (Sepharose Bead Conjugate)
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Species:   Mouse
Applications:   IP
Immunogen Range:   A synthetic phosphopeptide corresponding to residues around Thr180/Tyr182 of human p38 MAP kinase
Clonality:   Monoclonal Antibody
Isotype:   IgG1
GENE ID:   1432/5600/5603/6300
Swiss Prot:   Q16539/Q15759/O15264/P53778
Synonyms:   /
Purification:   Affinity purification
Storage:   Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Background:   p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).
Caculated MW:   40 kDa
Observed MW:   Refer to Figures
Applications:  
Reacitivity:   Human, Monkey, Mouse, Rat, S. cerevisiae
For research use only. Not intended for diagnostic or therapeutic use!
Additional information