ARM9019P-21 [Monoclonal Antibody]
Phospho-MPZL1-Tyr241 Rabbit Monoclonal Antibody
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Species:   Rabbit
Applications:   WB IP IF FC
Immunogen Range:   A synthetic peptide corresponding to residues surrounding Tyr241 of human PZR protein
Clonality:   Monoclonal Antibody
Isotype:   IgG
GENE ID:   9019
Swiss Prot:   O95297
Synonyms:   FLJ21047, PZR, PZR1b, PZRa, PZRb
Purification:   Affinity purification
Storage:   Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Background:   PZR (Protein zero related) is an immunoglobulin superfamily protein that specifically binds the tyrosine phosphatase SHP-2 through its intracellular immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (1,2). PZR is phosphorylated by c-Src, c-Fyn, c-Lyn, Csk, and c-Abl (3). PP1, a Src family kinase inhibitor, inhibits PZR phosphorylation (4,5). There are three alternatively spliced isoforms, designated as PZR, PZRa, and PZRb; both PZRa and PZRb lack ITIMs (6,7). PZR is the main receptor of ConA and has an important role in cell signaling via c-Src (4). PZR is expressed in many cell types and is localized to cell contacts and intracellular granules in BAECs and mesothelioma (REN) cells. PZR has been implicated as a cell adhesion protein that may be involved in SHP-2-dependent signaling at interendothelial cell contacts (3). Hypertyrosine phosphorylation of PZR was observed during embryogenesis in a mouse model of Noonan syndrome (8).Upon Con A treatment or H2O2 treatment, two PZR intracellular ITIM tyrosine sites-Tyr241 and Tyr263 are phosphorylated (4,8). Phosphorylation of these two sites facilitates recruitment of SHP-2 to PZR which alters the phosphatase activity of SHP-2 and affects its downstream signaling (5,8,9).
Caculated MW:   30-50 kDa
Observed MW:   Refer to Figures
Applications:   WB 1:1000
IF 1:800
FC 1:800
Reacitivity:   Bovine, Human, Mouse, Rat
For research use only. Not intended for diagnostic or therapeutic use!
Additional information