ARM3726-21 [Monoclonal Antibody]
Phospho-JUNB-Thr102/Thr104 Rabbit Monoclonal Antibody
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Species:   Rabbit
Applications:   WB IP
Immunogen Range:   A synthetic phosphopeptides corresponding to residues surrounding Thr102 and Thr104 of human JunB protein
Clonality:   Monoclonal Antibody
Isotype:   IgG
GENE ID:   3726
Swiss Prot:   P17275
Synonyms:   AP-1
Purification:   Affinity purification
Storage:   Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Background:   JunB is a basic region, leucine zipper (bZIP) transcription factor belonging to the Jun family that includes c-Jun and JunD. Jun family members homodimerize or heterodimerize with Fos and ATF proteins to form a functional transcription factor AP-1 (activator protein 1), whose activity is regulated by a variety of physiological and pathological stimuli such as growth factors, infections, and stress signals (1-4). While JunB sometimes antagonizes c-Jun transcriptional activity, it may functionally substitute for c-Jun during development in mice (5-7). JunB regulates hematopoietic stem cell number and plays an important role in the pathogenesis of chronic myelogenous leukemia (CML) and acute myeloid leukemia (AML) (8,9).JunB expression is selectively induced in T helper 2 (Th2) cells during T cell differentiation. JunB interacts with c-Maf, and the resulting complex functions synergistically to activate transcription of Interleukin-4 (IL-4), one of the signature cytokines secreted by Th2 cells. Transcriptional regulation of IL-4 was shown to be enhanced by JNK-mediated phosphorylation of JunB at Thr102 and Thr104 (10). Phosphorylation of these residues enhances DNA binding of the JunB/c-Maf complex at the P1 regulatory site of the IL-4 promoter, leading to Th2-restricted IL-4 expression.
Caculated MW:   43 kDa
Observed MW:   Refer to Figures
Applications:   WB 1:1000
Reacitivity:   Human
For research use only. Not intended for diagnostic or therapeutic use!
Additional information