ARM1978P-24C [Monoclonal Antibody]
Phospho-EIF4EBP1-Thr37/46 Rabbit Monoclonal Antibody (PE Conjugate)
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Species:   Rabbit
Applications:   FC
Immunogen Range:   A synthetic phosphopeptide corresponding to residues surrounding Thr37 and Thr46 of mouse 4E-BP1
Clonality:   Monoclonal Antibody
Isotype:   IgG
GENE ID:   1978
Swiss Prot:   Q13541
Synonyms:   4E-BP1, 4EBP1, BP-1, MGC4316, PHAS-I
Purification:   Affinity purification
Storage:   Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Background:   Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).
Caculated MW:   /
Observed MW:   Refer to Figures
Applications:   FC 1:50
Reacitivity:   D. melanogaster, Human, Monkey, Mouse, Rat
For research use only. Not intended for diagnostic or therapeutic use!
Additional information