AMM25491P-21C [Monoclonal Antibody]
NES Mouse Monoclonal Antibody (Alexa Fluor(R) 488 Conjugate)
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Species:   Mouse
Applications:   IF
Immunogen Range:   E15 rat spinal cord
Clonality:   Monoclonal Antibody
Isotype:   IgG1
GENE ID:   25491
Swiss Prot:   P48681
Synonyms:   NES, FLJ21841, Nbla00170, Nestin
Purification:   Affinity purification
Storage:   Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Background:   Nestin is an intermediate filament family member protein that is structurally related to the neurofilament proteins (1). It is highly expressed in the developing brain, where it may help to regulate cell structure and intracellular processes required for neural cell division and migration (1,2). Upon maturation of the brain, nestin expression is quickly down-regulated and replaced by expression of the neurofilament proteins (2). Because nestin is expressed in both mature and precursor neuronal and glial cells, as well as in the developing brain and in the brain and spinal cord following damage, nestin is widely accepted as a marker of neural stem/progenitor cells (3). Research studies have shown that expression of nestin is also found in cells from various nervous system tumors, including gliomas, neuroblastomas, astrocytomas, and it is generally accepted as a marker for neural cancer stem cells (3). However, nestin expression has also been observed in astrocytes, retina, cardiac muscle, pancreas, and other tissues (3). Therefore, the acceptance of nestin as an exclusive marker of neural stem/progenitor cells is not unanimous.
Caculated MW:   177 kDa
Observed MW:   Refer to Figures
Applications:   IF 1:50
Reacitivity:   Rat
For research use only. Not intended for diagnostic or therapeutic use!
Additional information