ARM142C-23C [Monoclonal Antibody]
Cleaved PARP1 (Asp214) Rabbit Monoclonal Antibody (Alexa Fluor(R) 647 Conjugate)
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Species:   Rabbit
Applications:   IF FC
Immunogen Range:   A synthetic peptide corresponding to residues surrounding Asp214 in human PARP protein
Clonality:   Monoclonal Antibody
Isotype:   IgG
GENE ID:   142
Swiss Prot:   P09874
Synonyms:   PARP, PPOL, ADPRT, ARTD1, ADPRT1, PARP-1, ADPRT 1, pADPRT-1
Purification:   Affinity purification
Storage:   Store at -20°C in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Avoid freeze/thaw cycles.
Background:   PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
Caculated MW:   89 kDa
Observed MW:   Refer to Figures
Applications:   IF 1:50
FC 1:50
Reacitivity:   Human, Monkey
For research use only. Not intended for diagnostic or therapeutic use!
Additional information